CRISPR-U™ Gene Knockout Cell Line
Strategy
MYL9 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MYL9 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MYL9 |
| Gene id | 10398 |
| Organism | Homo sapiens |
| Official full symbol | myosin light chain 9 |
| Gene type | protein-coding |
| Also known as | LC20, MLC-2C, MLC2, MRLC1, MYRL2 |
| Summary | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MYL9-201 | ENST00000279022.7 | 2786 | 172aa | Protein coding | CCDS13276 | P24844-1 | NM_006097.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MYL9-202 | ENST00000346786.2 | 1024 | 118aa | Protein coding | CCDS13277 | P24844-2 | - | TSL:1, GENCODE basic, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MYL9 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MYL9 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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