CRISPR-U™ Gene Knockout Cell Line Strategy

MYL9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MYL9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MYL9
Gene id 10398
Organism Homo sapiens
Official full symbol myosin light chain 9
Gene type protein-coding
Also known as LC20, MLC-2C, MLC2, MRLC1, MYRL2
Summary Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 20
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MYL9-201 ENST00000279022.7 2786 172aa Protein coding CCDS13276 P24844-1 NM_006097.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MYL9-202 ENST00000346786.2 1024 118aa Protein coding CCDS13277 P24844-2 - TSL:1, GENCODE basic,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MYL9
This KO Strategy loading
Red Cotton™ Notes Gene MYL9 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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