CRISPR-U™ Gene Knockout Cell Line Strategy

TUBB3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TUBB3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TUBB3
Gene id 10381
Organism Homo sapiens
Official full symbol tubulin beta 3 class III
Gene type protein-coding
Also known as CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
Summary This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
Genomic regions Chromosome 16
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TUBB3-206 ENST00000554444.5 1978 378aa Protein coding CCDS56012 Q13509-2 - TSL:2, GENCODE basic,
TUBB3-201 ENST00000315491.12 1706 450aa Protein coding CCDS10988 Q13509-1 NM_006086.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TUBB3-205 ENST00000554336.5 903 118aa Protein coding - G3V2R8 - TSL:3, GENCODE basic,
TUBB3-210 ENST00000555810.5 767 189aa Protein coding - G3V2A3 - CDS 3' incomplete, TSL:3,
TUBB3-203 ENST00000553967.1 736 164aa Protein coding - G3V2N6 - TSL:2, GENCODE basic,
TUBB3-208 ENST00000555576.5 572 97aa Protein coding - G3V5W4 - TSL:4, GENCODE basic,
TUBB3-212 ENST00000556565.5 566 46aa Protein coding - G3V542 - CDS 3' incomplete, TSL:4,
TUBB3-209 ENST00000555609.5 1855 55aa Nonsense mediated decay - G3V3J6 - TSL:3,
TUBB3-211 ENST00000556536.5 925 148aa Nonsense mediated decay - G3V3R4 - TSL:3,
TUBB3-213 ENST00000557262.5 888 51aa Nonsense mediated decay - G3V4U2 - TSL:1,
TUBB3-214 ENST00000557490.5 806 87aa Nonsense mediated decay - G3V3W7 - TSL:5,
TUBB3-202 ENST00000553656.5 550 51aa Nonsense mediated decay - G3V4U2 - TSL:4,
TUBB3-204 ENST00000554116.5 542 No protein Processed transcript - - - TSL:4,
TUBB3-216 ENST00000680788.1 5062 No protein Retained intron - - - -
TUBB3-215 ENST00000680647.1 2921 No protein Retained intron - - - -
TUBB3-207 ENST00000554927.1 561 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TUBB3
This KO Strategy loading
Red Cotton™ Notes Gene TUBB3 had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×