CRISPR-U™ Gene Knockout Cell Line
Strategy
TUBB3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TUBB3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TUBB3 |
| Gene id | 10381 |
| Organism | Homo sapiens |
| Official full symbol | tubulin beta 3 class III |
| Gene type | protein-coding |
| Also known as | CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4 |
| Summary | This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TUBB3-206 | ENST00000554444.5 | 1978 | 378aa | Protein coding | CCDS56012 | Q13509-2 | - | TSL:2, GENCODE basic, |
| TUBB3-201 | ENST00000315491.12 | 1706 | 450aa | Protein coding | CCDS10988 | Q13509-1 | NM_006086.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TUBB3-205 | ENST00000554336.5 | 903 | 118aa | Protein coding | - | G3V2R8 | - | TSL:3, GENCODE basic, |
| TUBB3-210 | ENST00000555810.5 | 767 | 189aa | Protein coding | - | G3V2A3 | - | CDS 3' incomplete, TSL:3, |
| TUBB3-203 | ENST00000553967.1 | 736 | 164aa | Protein coding | - | G3V2N6 | - | TSL:2, GENCODE basic, |
| TUBB3-208 | ENST00000555576.5 | 572 | 97aa | Protein coding | - | G3V5W4 | - | TSL:4, GENCODE basic, |
| TUBB3-212 | ENST00000556565.5 | 566 | 46aa | Protein coding | - | G3V542 | - | CDS 3' incomplete, TSL:4, |
| TUBB3-209 | ENST00000555609.5 | 1855 | 55aa | Nonsense mediated decay | - | G3V3J6 | - | TSL:3, |
| TUBB3-211 | ENST00000556536.5 | 925 | 148aa | Nonsense mediated decay | - | G3V3R4 | - | TSL:3, |
| TUBB3-213 | ENST00000557262.5 | 888 | 51aa | Nonsense mediated decay | - | G3V4U2 | - | TSL:1, |
| TUBB3-214 | ENST00000557490.5 | 806 | 87aa | Nonsense mediated decay | - | G3V3W7 | - | TSL:5, |
| TUBB3-202 | ENST00000553656.5 | 550 | 51aa | Nonsense mediated decay | - | G3V4U2 | - | TSL:4, |
| TUBB3-204 | ENST00000554116.5 | 542 | No protein | Processed transcript | - | - | - | TSL:4, |
| TUBB3-216 | ENST00000680788.1 | 5062 | No protein | Retained intron | - | - | - | - |
| TUBB3-215 | ENST00000680647.1 | 2921 | No protein | Retained intron | - | - | - | - |
| TUBB3-207 | ENST00000554927.1 | 561 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TUBB3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TUBB3 had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: