CRISPR-U™ Gene Knockout Cell Line Strategy

IRF9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IRF9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IRF9
Gene id 10379
Organism Homo sapiens
Official full symbol interferon regulatory factor 9
Gene type protein-coding
Also known as IRF-9, ISGF3, ISGF3G, p48
Summary This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Mutations in this gene result in Immunodeficiency 65.
Genomic regions Chromosome 14
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IRF9-202 ENST00000396864.8 1626 393aa Protein coding CCDS9615 Q00978 NM_006084.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IRF9-203 ENST00000557894.5 1391 353aa Protein coding - H0YMB0 - TSL:5, GENCODE basic,
IRF9-207 ENST00000560275.5 841 267aa Protein coding - H0YNP4 - CDS 3' incomplete, TSL:5,
IRF9-201 ENST00000324076.4 658 209aa Protein coding - H7BXP4 - CDS 5' incomplete, TSL:5,
IRF9-205 ENST00000559284.5 583 178aa Protein coding - H0YMB6 - CDS 3' incomplete, TSL:5,
IRF9-213 ENST00000561342.5 1066 50aa Nonsense mediated decay - H0YNM9 - TSL:5,
IRF9-211 ENST00000560852.1 731 50aa Nonsense mediated decay - H0YNM9 - TSL:3,
IRF9-215 ENST00000561415.1 1769 No protein Retained intron - - - TSL:2,
IRF9-210 ENST00000560542.1 819 No protein Retained intron - - - TSL:2,
IRF9-208 ENST00000560311.1 648 No protein Retained intron - - - TSL:2,
IRF9-209 ENST00000560365.5 623 No protein Retained intron - - - TSL:2,
IRF9-214 ENST00000561412.1 608 No protein Retained intron - - - TSL:5,
IRF9-206 ENST00000559863.1 573 No protein Retained intron - - - TSL:3,
IRF9-204 ENST00000559229.1 567 No protein Retained intron - - - TSL:2,
IRF9-212 ENST00000561009.5 546 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IRF9
This KO Strategy loading
Red Cotton™ Notes Gene IRF9 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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