CRISPR-U™ Gene Knockout Cell Line
Strategy
IRF9 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IRF9 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IRF9 |
| Gene id | 10379 |
| Organism | Homo sapiens |
| Official full symbol | interferon regulatory factor 9 |
| Gene type | protein-coding |
| Also known as | IRF-9, ISGF3, ISGF3G, p48 |
| Summary | This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Mutations in this gene result in Immunodeficiency 65. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IRF9-202 | ENST00000396864.8 | 1626 | 393aa | Protein coding | CCDS9615 | Q00978 | NM_006084.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IRF9-203 | ENST00000557894.5 | 1391 | 353aa | Protein coding | - | H0YMB0 | - | TSL:5, GENCODE basic, |
| IRF9-207 | ENST00000560275.5 | 841 | 267aa | Protein coding | - | H0YNP4 | - | CDS 3' incomplete, TSL:5, |
| IRF9-201 | ENST00000324076.4 | 658 | 209aa | Protein coding | - | H7BXP4 | - | CDS 5' incomplete, TSL:5, |
| IRF9-205 | ENST00000559284.5 | 583 | 178aa | Protein coding | - | H0YMB6 | - | CDS 3' incomplete, TSL:5, |
| IRF9-213 | ENST00000561342.5 | 1066 | 50aa | Nonsense mediated decay | - | H0YNM9 | - | TSL:5, |
| IRF9-211 | ENST00000560852.1 | 731 | 50aa | Nonsense mediated decay | - | H0YNM9 | - | TSL:3, |
| IRF9-215 | ENST00000561415.1 | 1769 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF9-210 | ENST00000560542.1 | 819 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF9-208 | ENST00000560311.1 | 648 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF9-209 | ENST00000560365.5 | 623 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF9-214 | ENST00000561412.1 | 608 | No protein | Retained intron | - | - | - | TSL:5, |
| IRF9-206 | ENST00000559863.1 | 573 | No protein | Retained intron | - | - | - | TSL:3, |
| IRF9-204 | ENST00000559229.1 | 567 | No protein | Retained intron | - | - | - | TSL:2, |
| IRF9-212 | ENST00000561009.5 | 546 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IRF9 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IRF9 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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