CRISPR-U™ Gene Knockout Cell Line
Strategy
TFG Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TFG Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TFG |
| Gene id | 10342 |
| Organism | Homo sapiens |
| Official full symbol | trafficking from ER to golgi regulator |
| Gene type | protein-coding |
| Also known as | HMSNP, SPG57, TF6, TRKT3 |
| Summary | There are several documented fusion oncoproteins encoded partially by this gene. This gene also participates in several oncogenic rearrangements resulting in anaplastic lymphoma and mixoid chondrosarcoma, and may play a role in the NF-kappaB pathway. Multiple transcript variants have been found for this gene. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 27 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TFG-211 | ENST00000674615.1 | 2037 | 400aa | Protein coding | CCDS2939 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-214 | ENST00000674758.1 | 2015 | 396aa | Protein coding | CCDS56266 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-207 | ENST00000487505.6 | 2005 | 400aa | Protein coding | CCDS2939 | C9JJP5 | - | TSL:3, GENCODE basic, APPRIS ALT1, |
| TFG-212 | ENST00000674645.1 | 1960 | 396aa | Protein coding | CCDS56266 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-220 | ENST00000675420.1 | 1921 | 396aa | Protein coding | CCDS56266 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-201 | ENST00000240851.9 | 1907 | 400aa | Protein coding | CCDS2939 | Q92734-1 | NM_006070.6 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| TFG-223 | ENST00000675553.1 | 1906 | 400aa | Protein coding | CCDS2939 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-234 | ENST00000676395.1 | 1888 | 400aa | Protein coding | CCDS2939 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-203 | ENST00000463568.6 | 1876 | 396aa | Protein coding | CCDS56266 | C9JUE0 | - | TSL:3, GENCODE basic, APPRIS ALT1, |
| TFG-218 | ENST00000675243.1 | 1854 | 400aa | Protein coding | CCDS2939 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-208 | ENST00000490574.6 | 1816 | 400aa | Protein coding | CCDS2939 | Q92734-1 | - | TSL:3, GENCODE basic, APPRIS P4, |
| TFG-221 | ENST00000675499.1 | 1805 | 400aa | Protein coding | CCDS2939 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-217 | ENST00000675047.1 | 1804 | 396aa | Protein coding | CCDS56266 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-235 | ENST00000676431.1 | 1793 | 396aa | Protein coding | CCDS56266 | - | - | GENCODE basic, APPRIS ALT1, |
| TFG-204 | ENST00000476228.5 | 1783 | 396aa | Protein coding | CCDS56266 | Q92734-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| TFG-210 | ENST00000620299.5 | 2028 | 284aa | Protein coding | - | Q92734-3 | - | TSL:5, GENCODE basic, |
| TFG-226 | ENST00000675692.1 | 2008 | 412aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-224 | ENST00000675586.1 | 2001 | 280aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-231 | ENST00000676111.1 | 1978 | 280aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-219 | ENST00000675246.1 | 1968 | 369aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-228 | ENST00000675958.1 | 1967 | 274aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-227 | ENST00000675890.1 | 1955 | 270aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-222 | ENST00000675543.1 | 1857 | 284aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-233 | ENST00000676308.1 | 1845 | 280aa | Protein coding | - | - | - | GENCODE basic, |
| TFG-209 | ENST00000615993.2 | 1759 | 280aa | Protein coding | - | Q92734-3 | - | TSL:1, GENCODE basic, |
| TFG-202 | ENST00000418917.7 | 1753 | 274aa | Protein coding | - | Q92734-2 | - | TSL:3, GENCODE basic, |
| TFG-205 | ENST00000479672.6 | 1712 | 141aa | Protein coding | - | C9JTY3 | - | TSL:3, GENCODE basic, |
| TFG-225 | ENST00000675591.1 | 2087 | 194aa | Nonsense mediated decay | - | - | - | - |
| TFG-230 | ENST00000676054.1 | 2066 | 278aa | Nonsense mediated decay | - | - | - | - |
| TFG-232 | ENST00000676276.1 | 2064 | 285aa | Nonsense mediated decay | - | - | - | - |
| TFG-216 | ENST00000675011.1 | 2017 | 249aa | Nonsense mediated decay | - | - | - | - |
| TFG-213 | ENST00000674699.1 | 1888 | 278aa | Nonsense mediated decay | - | - | - | - |
| TFG-236 | ENST00000676455.1 | 977 | 264aa | Nonsense mediated decay | - | - | - | - |
| TFG-229 | ENST00000676010.1 | 5864 | No protein | Retained intron | - | - | - | - |
| TFG-215 | ENST00000674798.1 | 4460 | No protein | Retained intron | - | - | - | - |
| TFG-206 | ENST00000481203.1 | 3271 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TFG |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TFG had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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