CRISPR-U™ Gene Knockout Cell Line
Strategy
CNPY2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CNPY2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CNPY2 |
| Gene id | 10330 |
| Organism | Homo sapiens |
| Official full symbol | canopy FGF signaling regulator 2 |
| Gene type | protein-coding |
| Also known as | HP10390, MSAP, TMEM4, ZSIG9 |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CNPY2-201 | ENST00000273308.9 | 1450 | 182aa | Protein coding | CCDS8914 | Q9Y2B0-1 | NM_014255.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CNPY2-209 | ENST00000551475.5 | 674 | 168aa | Protein coding | - | F8VXJ7 | - | CDS 3' incomplete, TSL:5, |
| CNPY2-208 | ENST00000551286.1 | 484 | 102aa | Protein coding | - | F8W1K5 | - | CDS 3' incomplete, TSL:5, |
| CNPY2-203 | ENST00000546466.5 | 673 | 80aa | Nonsense mediated decay | - | H0YI18 | - | CDS 5' incomplete, TSL:3, |
| CNPY2-212 | ENST00000553191.1 | 621 | 41aa | Nonsense mediated decay | - | H0YIH9 | - | CDS 5' incomplete, TSL:2, |
| CNPY2-210 | ENST00000551720.5 | 586 | No protein | Processed transcript | - | - | - | TSL:3, |
| CNPY2-207 | ENST00000551276.1 | 556 | No protein | Processed transcript | - | - | - | TSL:5, |
| CNPY2-204 | ENST00000546937.5 | 984 | No protein | Retained intron | - | - | - | TSL:2, |
| CNPY2-206 | ENST00000548013.5 | 817 | No protein | Retained intron | - | - | - | TSL:1, |
| CNPY2-202 | ENST00000546388.1 | 670 | No protein | Retained intron | - | - | - | TSL:1, |
| CNPY2-211 | ENST00000553164.1 | 666 | No protein | Retained intron | - | - | - | TSL:5, |
| CNPY2-205 | ENST00000547570.5 | 624 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CNPY2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CNPY2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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