CRISPR-U™ Gene Knockout Cell Line Strategy

EMC8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EMC8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EMC8
Gene id 10328
Organism Homo sapiens
Official full symbol ER membrane protein complex subunit 8
Gene type protein-coding
Also known as C16orf2, C16orf4, COX4NB, FAM158B, NOC4
Genomic regions Chromosome 16
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EMC8-201 ENST00000253457.8 1966 210aa Protein coding CCDS10954 O43402-1 NM_006067.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
EMC8-202 ENST00000435200.2 1935 126aa Protein coding CCDS45541 O43402-2 - TSL:2, GENCODE basic,
EMC8-203 ENST00000595980.5 556 100aa Nonsense mediated decay - M0R1B0 - CDS 5' incomplete, TSL:2,
EMC8-205 ENST00000597291.1 1826 No protein Retained intron - - - TSL:NA,
EMC8-206 ENST00000600807.1 851 No protein Retained intron - - - TSL:2,
EMC8-204 ENST00000596852.1 486 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EMC8
This KO Strategy loading
Red Cotton™ Notes Gene EMC8 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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