CRISPR-U™ Gene Knockout Cell Line
Strategy
EMC8 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EMC8 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EMC8 |
| Gene id | 10328 |
| Organism | Homo sapiens |
| Official full symbol | ER membrane protein complex subunit 8 |
| Gene type | protein-coding |
| Also known as | C16orf2, C16orf4, COX4NB, FAM158B, NOC4 |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EMC8-201 | ENST00000253457.8 | 1966 | 210aa | Protein coding | CCDS10954 | O43402-1 | NM_006067.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EMC8-202 | ENST00000435200.2 | 1935 | 126aa | Protein coding | CCDS45541 | O43402-2 | - | TSL:2, GENCODE basic, |
| EMC8-203 | ENST00000595980.5 | 556 | 100aa | Nonsense mediated decay | - | M0R1B0 | - | CDS 5' incomplete, TSL:2, |
| EMC8-205 | ENST00000597291.1 | 1826 | No protein | Retained intron | - | - | - | TSL:NA, |
| EMC8-206 | ENST00000600807.1 | 851 | No protein | Retained intron | - | - | - | TSL:2, |
| EMC8-204 | ENST00000596852.1 | 486 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EMC8 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EMC8 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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