CRISPR-U™ Gene Knockout Cell Line
Strategy
LANCL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LANCL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LANCL1 |
| Gene id | 10314 |
| Organism | Homo sapiens |
| Official full symbol | LanC like 1 |
| Gene type | protein-coding |
| Also known as | GPR69A, p40 |
| Summary | This gene encodes a loosely associated peripheral membrane protein related to the LanC family of bacterial membrane-associated proteins involved in the biosynthesis of antimicrobial peptides. This protein may play a role as a peptide-modifying enzyme component in eukaryotic cells. Previously considered a member of the G-protein-coupled receptor superfamily, this protein is now in the LanC family. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LANCL1-205 | ENST00000443314.5 | 4781 | 399aa | Protein coding | CCDS2392 | O43813 Q53TN2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| LANCL1-207 | ENST00000450366.7 | 4592 | 399aa | Protein coding | CCDS2392 | O43813 Q53TN2 | NM_006055.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LANCL1-204 | ENST00000441020.7 | 4503 | 399aa | Protein coding | CCDS2392 | O43813 Q53TN2 | - | TSL:5, GENCODE basic, APPRIS P1, |
| LANCL1-201 | ENST00000233714.8 | 1647 | 399aa | Protein coding | CCDS2392 | O43813 Q53TN2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| LANCL1-203 | ENST00000431941.6 | 1270 | 399aa | Protein coding | CCDS2392 | O43813 Q53TN2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| LANCL1-206 | ENST00000448951.5 | 808 | 196aa | Protein coding | - | E9PHS0 | - | CDS 3' incomplete, TSL:3, |
| LANCL1-202 | ENST00000412863.1 | 441 | 147aa | Protein coding | - | H7C2E3 | - | CDS 5' and 3' incomplete, TSL:5, |
| LANCL1-208 | ENST00000453956.1 | 590 | 82aa | Nonsense mediated decay | - | F8WDS9 | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LANCL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LANCL1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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