CRISPR-U™ Gene Knockout Cell Line
Strategy
CDKN2C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDKN2C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDKN2C |
| Gene id | 1031 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase inhibitor 2C |
| Gene type | protein-coding |
| Also known as | INK4C, p18, p18-INK4C |
| Summary | The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDKN2C-201 | ENST00000262662.5 | 2904 | 168aa | Protein coding | CCDS555 | P42773 Q6ICV4 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CDKN2C-203 | ENST00000396148.2 | 2076 | 168aa | Protein coding | CCDS555 | P42773 Q6ICV4 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CDKN2C-202 | ENST00000371761.4 | 995 | 168aa | Protein coding | CCDS555 | P42773 Q6ICV4 | NM_078626.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDKN2C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDKN2C had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: