CRISPR-U™ Gene Knockout Cell Line Strategy

CDKN2C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDKN2C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDKN2C
Gene id 1031
Organism Homo sapiens
Official full symbol cyclin dependent kinase inhibitor 2C
Gene type protein-coding
Also known as INK4C, p18, p18-INK4C
Summary The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDKN2C-201 ENST00000262662.5 2904 168aa Protein coding CCDS555 P42773 Q6ICV4 - TSL:2, GENCODE basic, APPRIS P1,
CDKN2C-203 ENST00000396148.2 2076 168aa Protein coding CCDS555 P42773 Q6ICV4 - TSL:1, GENCODE basic, APPRIS P1,
CDKN2C-202 ENST00000371761.4 995 168aa Protein coding CCDS555 P42773 Q6ICV4 NM_078626.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDKN2C
This KO Strategy loading
Red Cotton™ Notes Gene CDKN2C had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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