CRISPR-U™ Gene Knockout Cell Line Strategy

CDKN2A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDKN2A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDKN2A
Gene id 1029
Organism Homo sapiens
Official full symbol cyclin dependent kinase inhibitor 2A
Gene type protein-coding
Also known as ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16
Summary This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Genomic regions Chromosome 9
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDKN2A-214 ENST00000579755.2 1052 132aa Protein coding CCDS6511 Q8N726-1 NM_058195.4 TSL:1, GENCODE basic, MANE Plus Clinical v0.92,
CDKN2A-206 ENST00000494262.5 989 105aa Protein coding CCDS87644 P42771-2 - TSL:3, GENCODE basic,
CDKN2A-201 ENST00000304494.10 978 156aa Protein coding CCDS6510 P42771-1 NM_000077.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CDKN2A-209 ENST00000498628.6 926 105aa Protein coding CCDS87644 P42771-2 - TSL:2, GENCODE basic,
CDKN2A-208 ENST00000498124.1 880 167aa Protein coding CCDS56565 P42771-4 - TSL:1, GENCODE basic, APPRIS ALT2,
CDKN2A-210 ENST00000530628.2 748 132aa Protein coding CCDS6511 Q8N726-1 - TSL:5, GENCODE basic,
CDKN2A-212 ENST00000578845.2 678 105aa Protein coding CCDS87644 P42771-2 - TSL:2, GENCODE basic,
CDKN2A-213 ENST00000579122.1 666 138aa Protein coding - J3QRG6 - TSL:3, GENCODE basic,
CDKN2A-207 ENST00000497750.1 565 104aa Protein coding - K7ES20 - TSL:4, GENCODE basic,
CDKN2A-205 ENST00000479692.2 544 121aa Protein coding - K7ENC6 - TSL:5, GENCODE basic,
CDKN2A-203 ENST00000380151.3 794 116aa Nonsense mediated decay - P42771-3 - TSL:1,
CDKN2A-204 ENST00000470819.2 856 No protein Processed transcript - - - TSL:3,
CDKN2A-202 ENST00000380150.2 493 No protein Processed transcript - - - TSL:3,
CDKN2A-211 ENST00000577854.1 442 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDKN2A
This KO Strategy loading
Red Cotton™ Notes Gene CDKN2A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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