CRISPR-U™ Gene Knockout Cell Line Strategy

CDKN1A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDKN1A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDKN1A
Gene id 1026
Organism Homo sapiens
Official full symbol cyclin dependent kinase inhibitor 1A
Gene type protein-coding
Also known as CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
Summary This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDKN1A-203 ENST00000405375.5 2267 164aa Protein coding CCDS4824 A0A024RCX5 P38936 - TSL:1, GENCODE basic, APPRIS P1,
CDKN1A-201 ENST00000244741.10 2117 164aa Protein coding CCDS4824 A0A024RCX5 P38936 NM_000389.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDKN1A-204 ENST00000448526.6 2104 164aa Protein coding CCDS4824 A0A024RCX5 P38936 - TSL:3, GENCODE basic, APPRIS P1,
CDKN1A-208 ENST00000615513.4 2102 164aa Protein coding CCDS4824 A0A024RCX5 P38936 - TSL:2, GENCODE basic, APPRIS P1,
CDKN1A-202 ENST00000373711.3 793 164aa Protein coding CCDS4824 A0A024RCX5 P38936 - TSL:5, GENCODE basic, APPRIS P1,
CDKN1A-207 ENST00000478800.1 616 No protein Processed transcript - - - TSL:2,
CDKN1A-205 ENST00000459970.1 604 No protein Processed transcript - - - TSL:5,
CDKN1A-206 ENST00000462537.3 553 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDKN1A
This KO Strategy loading
Red Cotton™ Notes Gene CDKN1A had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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