CRISPR-U™ Gene Knockout Cell Line
Strategy
CDKN1A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDKN1A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDKN1A |
| Gene id | 1026 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase inhibitor 1A |
| Gene type | protein-coding |
| Also known as | CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1 |
| Summary | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDKN1A-203 | ENST00000405375.5 | 2267 | 164aa | Protein coding | CCDS4824 | A0A024RCX5 P38936 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CDKN1A-201 | ENST00000244741.10 | 2117 | 164aa | Protein coding | CCDS4824 | A0A024RCX5 P38936 | NM_000389.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CDKN1A-204 | ENST00000448526.6 | 2104 | 164aa | Protein coding | CCDS4824 | A0A024RCX5 P38936 | - | TSL:3, GENCODE basic, APPRIS P1, |
| CDKN1A-208 | ENST00000615513.4 | 2102 | 164aa | Protein coding | CCDS4824 | A0A024RCX5 P38936 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CDKN1A-202 | ENST00000373711.3 | 793 | 164aa | Protein coding | CCDS4824 | A0A024RCX5 P38936 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CDKN1A-207 | ENST00000478800.1 | 616 | No protein | Processed transcript | - | - | - | TSL:2, |
| CDKN1A-205 | ENST00000459970.1 | 604 | No protein | Processed transcript | - | - | - | TSL:5, |
| CDKN1A-206 | ENST00000462537.3 | 553 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDKN1A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDKN1A had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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