CRISPR-U™ Gene Knockout Cell Line Strategy

CDK8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDK8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDK8
Gene id 1024
Organism Homo sapiens
Official full symbol cyclin dependent kinase 8
Gene type protein-coding
Also known as IDDHBA, K35
Summary This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 13
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDK8-201 ENST00000381527.8 3065 464aa Protein coding CCDS9317 P49336-1 NM_001260.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDK8-206 ENST00000625988.1 795 148aa Protein coding - A0A0D9SEP3 - CDS 5' incomplete, TSL:5,
CDK8-202 ENST00000465820.6 586 195aa Protein coding - A0A0D9SFZ2 - CDS 5' and 3' incomplete, TSL:3,
CDK8-205 ENST00000536792.5 3032 164aa Nonsense mediated decay - F5H6D4 - TSL:1,
CDK8-204 ENST00000480323.1 1996 No protein Retained intron - - - TSL:2,
CDK8-203 ENST00000477277.5 1770 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDK8
This KO Strategy loading
Red Cotton™ Notes Gene CDK8 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: