CRISPR-U™ Gene Knockout Cell Line
Strategy
HNRNPR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HNRNPR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HNRNPR |
| Gene id | 10236 |
| Organism | Homo sapiens |
| Official full symbol | heterogeneous nuclear ribonucleoprotein R |
| Gene type | protein-coding |
| Also known as | HNRPR, hnRNP-R |
| Summary | This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HNRNPR-201 | ENST00000302271.11 | 7751 | 633aa | Protein coding | CCDS232 | O43390-1 | NM_005826.5 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| HNRNPR-210 | ENST00000478691.5 | 7646 | 535aa | Protein coding | CCDS60020 | O43390-4 | - | TSL:1, GENCODE basic, |
| HNRNPR-202 | ENST00000374612.5 | 2694 | 633aa | Protein coding | CCDS232 | O43390-1 | - | TSL:5, GENCODE basic, APPRIS P3, |
| HNRNPR-203 | ENST00000374616.7 | 2691 | 636aa | Protein coding | CCDS44085 | O43390-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| HNRNPR-212 | ENST00000606561.5 | 1837 | 494aa | Protein coding | CCDS72726 | B4DT28 | - | TSL:2, GENCODE basic, |
| HNRNPR-204 | ENST00000427764.3 | 1788 | 595aa | Protein coding | CCDS72727 | O43390-3 | - | TSL:1, GENCODE basic, |
| HNRNPR-214 | ENST00000675048.1 | 2099 | 481aa | Protein coding | - | - | - | GENCODE basic, |
| HNRNPR-208 | ENST00000476451.2 | 1032 | 238aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:4, |
| HNRNPR-205 | ENST00000463552.5 | 837 | 279aa | Protein coding | - | - | - | CDS 5' and 3' incomplete, TSL:4, |
| HNRNPR-207 | ENST00000470941.5 | 560 | 186aa | Protein coding | - | - | - | CDS 5' and 3' incomplete, TSL:4, |
| HNRNPR-213 | ENST00000641107.1 | 646 | 141aa | Nonsense mediated decay | - | A0A286YEZ8 | - | CDS 5' incomplete, |
| HNRNPR-209 | ENST00000476660.1 | 1637 | No protein | Processed transcript | - | - | - | TSL:1, |
| HNRNPR-206 | ENST00000464516.1 | 557 | No protein | Processed transcript | - | - | - | TSL:2, |
| HNRNPR-211 | ENST00000490652.1 | 612 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HNRNPR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HNRNPR had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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