CRISPR-U™ Gene Knockout Cell Line
Strategy
NME6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NME6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NME6 |
| Gene id | 10201 |
| Organism | Homo sapiens |
| Official full symbol | NME/NM23 nucleoside diphosphate kinase 6 |
| Gene type | protein-coding |
| Also known as | IPIA-ALPHA, NDK 6, NM23-H6 |
| Summary | Nucleoside diphosphate (NDP) kinases (EC 2.7.4.6), such as NME6, are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates (Mehus et al., 1999 ). |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 16 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NME6-213 | ENST00000451657.6 | 4776 | 163aa | Protein coding | CCDS77733 | O75414-3 | - | TSL:2, GENCODE basic, |
| NME6-209 | ENST00000442597.6 | 2903 | 186aa | Protein coding | CCDS77734 | O75414-1 | NM_001308426.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NME6-205 | ENST00000421967.5 | 2004 | 194aa | Protein coding | CCDS2763 | A0A0C4DG91 | - | TSL:1, GENCODE basic, |
| NME6-214 | ENST00000452211.5 | 1353 | 186aa | Protein coding | CCDS77734 | O75414-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| NME6-222 | ENST00000643457.1 | 1320 | 186aa | Protein coding | CCDS77734 | O75414-1 | - | GENCODE basic, APPRIS P1, |
| NME6-207 | ENST00000426689.6 | 1209 | 186aa | Protein coding | CCDS77734 | O75414-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NME6-202 | ENST00000415053.5 | 913 | 186aa | Protein coding | CCDS77734 | O75414-1 | - | TSL:3, GENCODE basic, APPRIS P1, |
| NME6-203 | ENST00000415644.5 | 849 | 119aa | Protein coding | CCDS82768 | O75414-2 | - | TSL:2, GENCODE basic, |
| NME6-211 | ENST00000447314.5 | 621 | 141aa | Protein coding | CCDS82767 | C9JQB1 | - | TSL:3, GENCODE basic, |
| NME6-208 | ENST00000435684.5 | 599 | 163aa | Protein coding | CCDS77733 | O75414-3 | - | TSL:2, GENCODE basic, |
| NME6-221 | ENST00000643011.1 | 2254 | 114aa | Protein coding | - | A0A2R8Y7V2 | - | GENCODE basic, |
| NME6-220 | ENST00000642710.1 | 939 | 88aa | Protein coding | - | A0A2R8Y547 | - | GENCODE basic, |
| NME6-210 | ENST00000444069.5 | 866 | 88aa | Protein coding | - | A0A2R8Y547 | - | TSL:1, GENCODE basic, |
| NME6-212 | ENST00000447724.1 | 668 | 61aa | Protein coding | - | C9J867 | - | CDS 3' incomplete, TSL:2, |
| NME6-216 | ENST00000456495.5 | 638 | 111aa | Protein coding | - | C9J1J2 | - | CDS 3' incomplete, TSL:5, |
| NME6-206 | ENST00000425930.5 | 613 | 174aa | Protein coding | - | C9J9V6 | - | CDS 3' incomplete, TSL:2, |
| NME6-204 | ENST00000418431.5 | 794 | 82aa | Nonsense mediated decay | - | F2Z2J2 | - | TSL:3, |
| NME6-215 | ENST00000454531.1 | 552 | 82aa | Nonsense mediated decay | - | F2Z2J2 | - | TSL:4, |
| NME6-218 | ENST00000494209.1 | 1268 | No protein | Retained intron | - | - | - | TSL:3, |
| NME6-217 | ENST00000484959.1 | 1091 | No protein | Retained intron | - | - | - | TSL:1, |
| NME6-201 | ENST00000302378.2 | 490 | No protein | Retained intron | - | - | - | TSL:4, |
| NME6-219 | ENST00000495734.1 | 401 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NME6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NME6 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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