CRISPR-U™ Gene Knockout Cell Line Strategy

CDK5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDK5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDK5
Gene id 1020
Organism Homo sapiens
Official full symbol cyclin dependent kinase 5
Gene type protein-coding
Also known as LIS7, PSSALRE
Summary This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 7
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDK5-204 ENST00000485972.6 1122 292aa Protein coding CCDS47748 Q00535-1 NM_004935.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDK5-201 ENST00000297518.4 783 260aa Protein coding CCDS55184 Q00535-2 - TSL:1, GENCODE basic,
CDK5-205 ENST00000487703.5 922 No protein Retained intron - - - TSL:5,
CDK5-203 ENST00000476691.1 726 No protein Retained intron - - - TSL:4,
CDK5-202 ENST00000469108.1 582 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDK5
This KO Strategy loading
Red Cotton™ Notes Gene CDK5 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×