CRISPR-U™ Gene Knockout Cell Line
Strategy
TXNDC9 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TXNDC9 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TXNDC9 |
| Gene id | 10190 |
| Organism | Homo sapiens |
| Official full symbol | thioredoxin domain containing 9 |
| Gene type | protein-coding |
| Also known as | APACD, PHLP3 |
| Summary | The protein encoded by this gene is a member of the thioredoxin family. The exact function of this protein is not known but it is associated with cell differentiation. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TXNDC9-201 | ENST00000264255.8 | 1508 | 226aa | Protein coding | CCDS2044 | O14530-1 | NM_005783.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TXNDC9-202 | ENST00000409434.5 | 1111 | 188aa | Protein coding | - | O14530-2 | - | TSL:1, GENCODE basic, |
| TXNDC9-205 | ENST00000434323.5 | 733 | 122aa | Protein coding | - | F8WCJ3 | - | TSL:5, GENCODE basic, |
| TXNDC9-203 | ENST00000409705.5 | 568 | 135aa | Protein coding | - | B8ZZX4 | - | CDS 3' incomplete, TSL:4, |
| TXNDC9-204 | ENST00000422767.6 | 768 | 64aa | Nonsense mediated decay | - | F8WBV5 | - | TSL:5, |
| TXNDC9-206 | ENST00000438680.1 | 739 | 74aa | Nonsense mediated decay | - | G3XAJ4 | - | TSL:2, |
| TXNDC9-209 | ENST00000477337.1 | 519 | No protein | Processed transcript | - | - | - | TSL:4, |
| TXNDC9-207 | ENST00000463385.5 | 1099 | No protein | Retained intron | - | - | - | TSL:2, |
| TXNDC9-208 | ENST00000465183.1 | 599 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TXNDC9 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TXNDC9 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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