CRISPR-U™ Gene Knockout Cell Line Strategy

TXNDC9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TXNDC9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TXNDC9
Gene id 10190
Organism Homo sapiens
Official full symbol thioredoxin domain containing 9
Gene type protein-coding
Also known as APACD, PHLP3
Summary The protein encoded by this gene is a member of the thioredoxin family. The exact function of this protein is not known but it is associated with cell differentiation.
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TXNDC9-201 ENST00000264255.8 1508 226aa Protein coding CCDS2044 O14530-1 NM_005783.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TXNDC9-202 ENST00000409434.5 1111 188aa Protein coding - O14530-2 - TSL:1, GENCODE basic,
TXNDC9-205 ENST00000434323.5 733 122aa Protein coding - F8WCJ3 - TSL:5, GENCODE basic,
TXNDC9-203 ENST00000409705.5 568 135aa Protein coding - B8ZZX4 - CDS 3' incomplete, TSL:4,
TXNDC9-204 ENST00000422767.6 768 64aa Nonsense mediated decay - F8WBV5 - TSL:5,
TXNDC9-206 ENST00000438680.1 739 74aa Nonsense mediated decay - G3XAJ4 - TSL:2,
TXNDC9-209 ENST00000477337.1 519 No protein Processed transcript - - - TSL:4,
TXNDC9-207 ENST00000463385.5 1099 No protein Retained intron - - - TSL:2,
TXNDC9-208 ENST00000465183.1 599 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TXNDC9
This KO Strategy loading
Red Cotton™ Notes Gene TXNDC9 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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