CRISPR-U™ Gene Knockout Cell Line
Strategy
CDK3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDK3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDK3 |
| Gene id | 1018 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase 3 |
| Gene type | protein-coding |
| Summary | This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDK3-201 | ENST00000425876.6 | 1582 | 305aa | Protein coding | CCDS11736 | Q00526 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CDK3-202 | ENST00000448471.2 | 1570 | 305aa | Protein coding | CCDS11736 | Q00526 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CDK3-203 | ENST00000586261.2 | 803 | 213aa | Protein coding | - | K7EJ83 | - | CDS 3' incomplete, TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDK3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDK3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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