CRISPR-U™ Gene Knockout Cell Line Strategy

SLC25A13 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SLC25A13 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SLC25A13
Gene id 10165
Organism Homo sapiens
Official full symbol solute carrier family 25 member 13
Gene type protein-coding
Summary This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 7
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SLC25A13-202 ENST00000416240.6 3192 676aa Protein coding CCDS55130 Q9UJS0-2 - TSL:1, GENCODE basic, APPRIS ALT1,
SLC25A13-201 ENST00000265631.10 3141 675aa Protein coding CCDS5645 Q9UJS0-1 NM_014251.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
SLC25A13-203 ENST00000472162.2 568 73aa Nonsense mediated decay - R4GN64 - TSL:4,
SLC25A13-208 ENST00000494085.1 766 No protein Processed transcript - - - TSL:2,
SLC25A13-206 ENST00000490072.5 578 No protein Processed transcript - - - TSL:5,
SLC25A13-204 ENST00000484495.5 540 No protein Processed transcript - - - TSL:5,
SLC25A13-207 ENST00000492869.1 433 No protein Processed transcript - - - TSL:5,
SLC25A13-205 ENST00000487710.1 201 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SLC25A13
This KO Strategy loading
Red Cotton™ Notes Gene SLC25A13 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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