CRISPR-U™ Gene Knockout Cell Line
Strategy
SLC25A13 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SLC25A13 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SLC25A13 |
| Gene id | 10165 |
| Organism | Homo sapiens |
| Official full symbol | solute carrier family 25 member 13 |
| Gene type | protein-coding |
| Also known as | ARALAR2, CITRIN, CTLN2, NICCD |
| Summary | This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SLC25A13-202 | ENST00000416240.6 | 3192 | 676aa | Protein coding | CCDS55130 | Q9UJS0-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| SLC25A13-201 | ENST00000265631.10 | 3141 | 675aa | Protein coding | CCDS5645 | Q9UJS0-1 | NM_014251.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| SLC25A13-203 | ENST00000472162.2 | 568 | 73aa | Nonsense mediated decay | - | R4GN64 | - | TSL:4, |
| SLC25A13-208 | ENST00000494085.1 | 766 | No protein | Processed transcript | - | - | - | TSL:2, |
| SLC25A13-206 | ENST00000490072.5 | 578 | No protein | Processed transcript | - | - | - | TSL:5, |
| SLC25A13-204 | ENST00000484495.5 | 540 | No protein | Processed transcript | - | - | - | TSL:5, |
| SLC25A13-207 | ENST00000492869.1 | 433 | No protein | Processed transcript | - | - | - | TSL:5, |
| SLC25A13-205 | ENST00000487710.1 | 201 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SLC25A13 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SLC25A13 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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