CRISPR-U™ Gene Knockout Cell Line Strategy

CTDSP2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CTDSP2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CTDSP2
Gene id 10106
Organism Homo sapiens
Official full symbol CTD small phosphatase 2
Gene type protein-coding
Also known as OS4, PSR2, SCP2
Genomic regions Chromosome 12
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CTDSP2-201 ENST00000398073.7 4785 271aa Protein coding CCDS41801 A0A024RBA6 O14595 NM_005730.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CTDSP2-204 ENST00000548823.1 1368 98aa Protein coding - F8W1I1 - TSL:1, GENCODE basic,
CTDSP2-203 ENST00000547701.5 988 119aa Protein coding - F8W184 - TSL:1, GENCODE basic,
CTDSP2-205 ENST00000549039.5 797 125aa Protein coding - H0YI29 - CDS 5' incomplete, TSL:2,
CTDSP2-206 ENST00000550144.1 544 182aa Protein coding - H0YI12 - CDS 5' and 3' incomplete, TSL:4,
CTDSP2-207 ENST00000551594.1 900 102aa Nonsense mediated decay - F8VYL0 - TSL:3,
CTDSP2-202 ENST00000547169.5 1340 No protein Retained intron - - - TSL:2,
CTDSP2-208 ENST00000552294.1 436 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CTDSP2
This KO Strategy loading
Red Cotton™ Notes Gene CTDSP2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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