CRISPR-U™ Gene Knockout Cell Line
Strategy
ABCF2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ABCF2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ABCF2 |
| Gene id | 10061 |
| Organism | Homo sapiens |
| Official full symbol | ATP binding cassette subfamily F member 2 |
| Gene type | protein-coding |
| Also known as | ABC28, EST133090, HUSSY-18, HUSSY18 |
| Summary | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Related pseudogenes have been identified on chromosomes 3 and 7. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ABCF2-201 | ENST00000287844.7 | 4527 | 623aa | Protein coding | CCDS5923 | Q9UG63-1 | NM_007189.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ABCF2-203 | ENST00000468073.5 | 1031 | 287aa | Protein coding | - | C9JZV3 | - | CDS 3' incomplete, TSL:2, |
| ABCF2-202 | ENST00000441774.1 | 941 | 240aa | Protein coding | - | C9JHK9 | - | CDS 3' incomplete, TSL:3, |
| ABCF2-204 | ENST00000473874.1 | 648 | No protein | Processed transcript | - | - | - | TSL:3, |
| ABCF2-205 | ENST00000477252.1 | 249 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ABCF2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ABCF2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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