CRISPR-U™ Gene Knockout Cell Line Strategy

ABCF2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ABCF2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ABCF2
Gene id 10061
Organism Homo sapiens
Official full symbol ATP binding cassette subfamily F member 2
Gene type protein-coding
Also known as ABC28, EST133090, HUSSY-18, HUSSY18
Summary This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Related pseudogenes have been identified on chromosomes 3 and 7.
Genomic regions Chromosome 7
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ABCF2-201 ENST00000287844.7 4527 623aa Protein coding CCDS5923 Q9UG63-1 NM_007189.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ABCF2-203 ENST00000468073.5 1031 287aa Protein coding - C9JZV3 - CDS 3' incomplete, TSL:2,
ABCF2-202 ENST00000441774.1 941 240aa Protein coding - C9JHK9 - CDS 3' incomplete, TSL:3,
ABCF2-204 ENST00000473874.1 648 No protein Processed transcript - - - TSL:3,
ABCF2-205 ENST00000477252.1 249 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ABCF2
This KO Strategy loading
Red Cotton™ Notes Gene ABCF2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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