CRISPR-U™ Gene Knockout Cell Line
Strategy
PARP3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARP3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PARP3 |
| Gene id | 10039 |
| Organism | Homo sapiens |
| Official full symbol | poly(ADP-ribose) polymerase family member 3 |
| Gene type | protein-coding |
| Also known as | ADPRT3, ADPRTL2, ADPRTL3, ARTD3, IRT1, PADPRT-3 |
| Summary | The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PARP3-202 | ENST00000417220.6 | 2472 | 533aa | Protein coding | CCDS43097 | Q9Y6F1-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PARP3-206 | ENST00000471971.6 | 2393 | 533aa | Protein coding | CCDS43097 | A0A024R318 F8WAY7 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PARP3-201 | ENST00000398755.8 | 2337 | 533aa | Protein coding | CCDS43097 | Q9Y6F1-2 | NM_001003931.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PARP3-203 | ENST00000431474.6 | 2096 | 533aa | Protein coding | CCDS43097 | Q9Y6F1-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PARP3-209 | ENST00000498510.2 | 1952 | 533aa | Protein coding | CCDS43097 | A0A024R318 C9J9C7 | - | TSL:2, GENCODE basic, APPRIS P1, |
| PARP3-208 | ENST00000486510.1 | 443 | No protein | Processed transcript | - | - | - | TSL:2, |
| PARP3-204 | ENST00000470601.5 | 940 | No protein | Retained intron | - | - | - | TSL:2, |
| PARP3-207 | ENST00000475782.1 | 771 | No protein | Retained intron | - | - | - | TSL:2, |
| PARP3-205 | ENST00000470749.1 | 697 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PARP3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PARP3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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