CRISPR-U™ Gene Knockout Cell Line Strategy

PARP3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARP3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARP3
Gene id 10039
Organism Homo sapiens
Official full symbol poly(ADP-ribose) polymerase family member 3
Gene type protein-coding
Also known as ADPRT3, ADPRTL2, ADPRTL3, ARTD3, IRT1, PADPRT-3
Summary The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 3
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARP3-202 ENST00000417220.6 2472 533aa Protein coding CCDS43097 Q9Y6F1-1 - TSL:5, GENCODE basic, APPRIS P1,
PARP3-206 ENST00000471971.6 2393 533aa Protein coding CCDS43097 A0A024R318 F8WAY7 - TSL:1, GENCODE basic, APPRIS P1,
PARP3-201 ENST00000398755.8 2337 533aa Protein coding CCDS43097 Q9Y6F1-2 NM_001003931.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PARP3-203 ENST00000431474.6 2096 533aa Protein coding CCDS43097 Q9Y6F1-1 - TSL:1, GENCODE basic, APPRIS P1,
PARP3-209 ENST00000498510.2 1952 533aa Protein coding CCDS43097 A0A024R318 C9J9C7 - TSL:2, GENCODE basic, APPRIS P1,
PARP3-208 ENST00000486510.1 443 No protein Processed transcript - - - TSL:2,
PARP3-204 ENST00000470601.5 940 No protein Retained intron - - - TSL:2,
PARP3-207 ENST00000475782.1 771 No protein Retained intron - - - TSL:2,
PARP3-205 ENST00000470749.1 697 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARP3
This KO Strategy loading
Red Cotton™ Notes Gene PARP3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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