CRISPR-U™ Gene Knockout Cell Line
Strategy
PARP2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARP2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PARP2 |
| Gene id | 10038 |
| Organism | Homo sapiens |
| Official full symbol | poly(ADP-ribose) polymerase 2 |
| Gene type | protein-coding |
| Also known as | ADPRT2, ADPRTL2, ADPRTL3, ARTD2, PARP-2, pADPRT-2 |
| Summary | This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PARP2-201 | ENST00000250416.9 | 1886 | 583aa | Protein coding | CCDS41910 | Q9UGN5-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PARP2-202 | ENST00000429687.8 | 1827 | 570aa | Protein coding | CCDS45077 | Q9UGN5-2 | NM_001042618.2 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PARP2-204 | ENST00000527915.5 | 1980 | 531aa | Protein coding | - | G3V167 | - | TSL:2, GENCODE basic, |
| PARP2-210 | ENST00000539930.1 | 635 | 177aa | Protein coding | - | H0YH02 | - | CDS 5' incomplete, TSL:3, |
| PARP2-206 | ENST00000529465.6 | 717 | 110aa | Nonsense mediated decay | - | E9PJ27 | - | TSL:4, |
| PARP2-211 | ENST00000555140.1 | 530 | No protein | Processed transcript | - | - | - | TSL:4, |
| PARP2-208 | ENST00000532299.5 | 876 | No protein | Retained intron | - | - | - | TSL:2, |
| PARP2-207 | ENST00000530598.2 | 842 | No protein | Retained intron | - | - | - | TSL:3, |
| PARP2-209 | ENST00000534664.1 | 566 | No protein | Retained intron | - | - | - | TSL:2, |
| PARP2-205 | ENST00000528465.2 | 551 | No protein | Retained intron | - | - | - | TSL:5, |
| PARP2-203 | ENST00000527384.1 | 429 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PARP2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PARP2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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