CRISPR-U™ Gene Knockout Cell Line Strategy

PARP2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PARP2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARP2
Gene id 10038
Organism Homo sapiens
Official full symbol poly(ADP-ribose) polymerase 2
Gene type protein-coding
Also known as ADPRT2, ADPRTL2, ADPRTL3, ARTD2, PARP-2, pADPRT-2
Summary This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
Genomic regions Chromosome 14
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARP2-201 ENST00000250416.9 1886 583aa Protein coding CCDS41910 Q9UGN5-1 - TSL:1, GENCODE basic, APPRIS ALT2,
PARP2-202 ENST00000429687.8 1827 570aa Protein coding CCDS45077 Q9UGN5-2 NM_001042618.2 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PARP2-204 ENST00000527915.5 1980 531aa Protein coding - G3V167 - TSL:2, GENCODE basic,
PARP2-210 ENST00000539930.1 635 177aa Protein coding - H0YH02 - CDS 5' incomplete, TSL:3,
PARP2-206 ENST00000529465.6 717 110aa Nonsense mediated decay - E9PJ27 - TSL:4,
PARP2-211 ENST00000555140.1 530 No protein Processed transcript - - - TSL:4,
PARP2-208 ENST00000532299.5 876 No protein Retained intron - - - TSL:2,
PARP2-207 ENST00000530598.2 842 No protein Retained intron - - - TSL:3,
PARP2-209 ENST00000534664.1 566 No protein Retained intron - - - TSL:2,
PARP2-205 ENST00000528465.2 551 No protein Retained intron - - - TSL:5,
PARP2-203 ENST00000527384.1 429 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARP2
This KO Strategy loading
Red Cotton™ Notes Gene PARP2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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