CRISPR-U™ Gene Knockout Cell Line Strategy

PDCD6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDCD6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDCD6
Gene id 10016
Organism Homo sapiens
Official full symbol programmed cell death 6
Gene type protein-coding
Also known as ALG-2, ALG2, PEF1B
Summary This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.
Genomic regions Chromosome 5
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDCD6-201 ENST00000264933.9 1110 191aa Protein coding CCDS3854 O75340-1 NM_013232.4 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
PDCD6-205 ENST00000507528.5 1088 189aa Protein coding CCDS58940 O75340-2 - TSL:1, GENCODE basic, APPRIS ALT1,
PDCD6-211 ENST00000614778.4 1050 121aa Protein coding CCDS75223 A0A024QZ42 - TSL:2, GENCODE basic,
PDCD6-212 ENST00000618970.4 931 123aa Protein coding CCDS75222 A0A087WZ38 - TSL:3, GENCODE basic,
PDCD6-202 ENST00000505221.5 672 69aa Protein coding CCDS58941 O75340-3 - TSL:3, GENCODE basic,
PDCD6-206 ENST00000509581.5 1156 55aa Protein coding - Q86W51 - TSL:1, GENCODE basic,
PDCD6-213 ENST00000628729.2 1073 39aa Protein coding - D6RA21 - TSL:5, GENCODE basic,
PDCD6-204 ENST00000507473.1 486 104aa Protein coding - H0Y9X3 - CDS 5' incomplete, TSL:3,
PDCD6-203 ENST00000505526.1 1484 39aa Nonsense mediated decay - D6RA21 - TSL:1,
PDCD6-207 ENST00000511482.1 878 No protein Processed transcript - - - TSL:5,
PDCD6-208 ENST00000512466.1 3763 No protein Retained intron - - - TSL:2,
PDCD6-209 ENST00000513582.5 3500 No protein Retained intron - - - TSL:2,
PDCD6-210 ENST00000515587.1 855 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDCD6
This KO Strategy loading
Red Cotton™ Notes Gene PDCD6 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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