CRISPR-U™ Gene Knockout Cell Line
Strategy
PDCD6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDCD6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDCD6 |
| Gene id | 10016 |
| Organism | Homo sapiens |
| Official full symbol | programmed cell death 6 |
| Gene type | protein-coding |
| Also known as | ALG-2, ALG2, PEF1B |
| Summary | This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDCD6-201 | ENST00000264933.9 | 1110 | 191aa | Protein coding | CCDS3854 | O75340-1 | NM_013232.4 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| PDCD6-205 | ENST00000507528.5 | 1088 | 189aa | Protein coding | CCDS58940 | O75340-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| PDCD6-211 | ENST00000614778.4 | 1050 | 121aa | Protein coding | CCDS75223 | A0A024QZ42 | - | TSL:2, GENCODE basic, |
| PDCD6-212 | ENST00000618970.4 | 931 | 123aa | Protein coding | CCDS75222 | A0A087WZ38 | - | TSL:3, GENCODE basic, |
| PDCD6-202 | ENST00000505221.5 | 672 | 69aa | Protein coding | CCDS58941 | O75340-3 | - | TSL:3, GENCODE basic, |
| PDCD6-206 | ENST00000509581.5 | 1156 | 55aa | Protein coding | - | Q86W51 | - | TSL:1, GENCODE basic, |
| PDCD6-213 | ENST00000628729.2 | 1073 | 39aa | Protein coding | - | D6RA21 | - | TSL:5, GENCODE basic, |
| PDCD6-204 | ENST00000507473.1 | 486 | 104aa | Protein coding | - | H0Y9X3 | - | CDS 5' incomplete, TSL:3, |
| PDCD6-203 | ENST00000505526.1 | 1484 | 39aa | Nonsense mediated decay | - | D6RA21 | - | TSL:1, |
| PDCD6-207 | ENST00000511482.1 | 878 | No protein | Processed transcript | - | - | - | TSL:5, |
| PDCD6-208 | ENST00000512466.1 | 3763 | No protein | Retained intron | - | - | - | TSL:2, |
| PDCD6-209 | ENST00000513582.5 | 3500 | No protein | Retained intron | - | - | - | TSL:2, |
| PDCD6-210 | ENST00000515587.1 | 855 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDCD6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDCD6 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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